2-Aminopyridine--a label for bridging of oligosaccharides HPLC profiling and glycoarray printing.
Journal: Glycoconj J
Date: 2008 Jan
Major Program(s) or Research Group(s): GLYCO
PubMed ID: 17674203
PMC ID: not available
Abstract: 2-Aminopyridine derivatives of oligosaccharides (OS-AP) were printed onto microchips by two different ways. The first method is based on direct covalent insertion of OS-AP in polyacrylamide gel 3D chip. The second method is based on conversion of OS-AP into more reactive OS-aminoalditol followed by covalent printing onto NHS-activated glass slides. This approach extends the range of saccharides suitable for covalent printing due to availability of commercial OS-AP and easy high-performance liquid chromatography separation of glycoprotein N-chains in form of AP derivatives.