2-Aminopyridine--a label for bridging of oligosaccharides HPLC profiling and glycoarray printing.

Author(s): Shilova NV,  Galanina OE,  Rubina AY,  Butvilovskaya VI,  Huflejt ME,  Chambers J,  Roucoux A,  Bovin NV

Journal: Glycoconj J

Date: 2008 Jan

Major Program(s) or Research Group(s): GLYCO

PubMed ID: 17674203

PMC ID: not available

Abstract: 2-Aminopyridine derivatives of oligosaccharides (OS-AP) were printed onto microchips by two different ways. The first method is based on direct covalent insertion of OS-AP in polyacrylamide gel 3D chip. The second method is based on conversion of OS-AP into more reactive OS-aminoalditol followed by covalent printing onto NHS-activated glass slides. This approach extends the range of saccharides suitable for covalent printing due to availability of commercial OS-AP and easy high-performance liquid chromatography separation of glycoprotein N-chains in form of AP derivatives.