Publications

Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer.

Author(s): Fisher WE,  Cruz-Monserrate Z,  McElhany AL,  Lesinski GB,  Hart PA,  Ghosh R,  Van Buren G,  Fishman DS,  Rinaudo JAS,  Serrano J,  Srivastava S,  Mace T,  Topazian M,  Feng Z,  Yadav D,  Pandol SJ,  Hughes SJ,  Liu RY,  Lu E,  Orr R,  Whitcomb DC,  Abouhamze AS,  Steen H,  Sellers ZM,  Troendle DM,  Uc A,  Lowe ME,  Conwell DL,  Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC)

Journal: Pancreas

Date: 2018 Nov-Dec

Major Program(s) or Research Group(s): CBRG

PubMed ID: 30325860

PMC ID: PMC6197069

Abstract: High-quality and well-annotated biorepositories are needed to better understand the pathophysiology and biologic mechanisms of chronic pancreatitis (CP) and its consequences. We report a methodology for the development of a robust standard operating procedure (SOP) for a biorepository based on the experience of the clinical centers within the consortium to study Chronic Pancreatitis, Diabetes and Pancreas Cancer Clinical Centers (CPDPC), supported by the National Cancer Institute and the National Institute for Diabetes and Digestive and Kidney Diseases as a unique multidisciplinary model to study CP, diabetes, and pancreatic cancer in both children and adults. Standard operating procedures from the CPDPC centers were evaluated and consolidated. The literature was reviewed for standard biorepository operating procedures that facilitated downstream molecular analysis. The existing literature on biobanking practices was harmonized with the SOPs from the clinical centers to produce a biorepository for pancreatic research. This article reports the methods and basic principles behind the creation of SOPs to develop a biorepository for the CPDPC. These will serve as a guide for investigators developing biorepositories in pancreas research. Rigorous and meticulous adherence to standardized biospecimen collection will facilitate investigations to better understand the pathophysiology and biologic mechanisms of CP, diabetes, and pancreatic cancer.