Targeted methods for quantitative analysis of protein glycosylation.
Journal: Proteomics Clin Appl
Date: 2015 Feb
Major Program(s) or Research Group(s): GLYCO
PubMed ID: 25522218
PMC ID: PMC5780646
Abstract: Quantification of proteins by LC-MS/MS-MRM has become a standard method with broad projected clinical applicability. MRM quantification of protein modifications is, however, far less utilized, especially in the case of glycoproteins. This review summarizes current methods for quantitative analysis of protein glycosylation with a focus on MRM methods. We describe advantages of this quantitative approach, analytical parameters that need to be optimized to achieve reliable measurements, and point out the limitations. Differences between major classes of N- and O-glycopeptides are described and class-specific glycopeptide assays are demonstrated.